KMID : 0352720150390020141
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Journal of Ginseng Research 2015 Volume.39 No. 2 p.141 ~ p.147
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Structural investigation of ginsenoside Rf with PPAR¥ã major transcriptional factor of adipogenesis and its impact on adipocyte
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Siraj Fayeza Md
Natarajan Sathishkumar Huq Md Amdadul Kim Yeon-Ju Yang Deok-Chun
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Abstract
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Background: Adipocytes, which are the main cellular component of adipose tissue, are the building blocks of obesity. The nuclear hormone receptor PPAR¥ã is a major regulator of adipocyte differentiation and development. Obesity, which is one of the most dangerous yet silent diseases of all time, is fast becoming a critical area of research focus. Methods: In this study, we initially aimed to investigate whether the ginsenoside Rf, a compound that is only present in Panax ginseng Meyer, interacts with PPAR¥ã by molecular docking simulations. After we performed the docking simulation the result has been analyzed with several different software programs, including Discovery Studio, Pymol, Chimera, Ligplus, and Pose View. All of the programs identified the same mechanism of interaction between PPAR¥ã and Rf, at the same active site. To determine the drug-like and biological activities of Rf, we calculate its absorption, distribution, metabolism, excretion, and toxic (ADMET) and prediction of activity spectra for substances (PASS) properties. Considering the results obtained from the computational investigations, the focus was on the in vitro experiments. Results: Because the docking simulations predicted the formation of structural bonds between Rf and PPAR¥ã, we also investigated whether any evidence for these bonds could be observed at the cellular level. These experiments revealed that Rf treatment of 3T3-L1 adipocytes downregulated the expression levels of PPAR¥ã and perilipin, and also decreased the amount of lipid accumulated at different doses. Conclusion: The ginsenoside Rf appears to be promising compound that could prove useful in antiobesity treatments.
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KEYWORD
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adipocyte, ginsenoside Rf, molecular docking, Panax ginseng, PPAR¥ã
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